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BioMed Research International
Volume 2014, Article ID 456323, 10 pages
http://dx.doi.org/10.1155/2014/456323
Research Article

MicroRNA Expression Profiling Altered by Variant Dosage of Radiation Exposure

1Institute of Medical Sciences, Tzu Chi University, No. 701, Zhongyang Road, Section 3, Hualien 97004, Taiwan
2Laboratory for Cytogenetics, Center for Genetic Counseling, Buddhist Tzu Chi General Hospital, Hualien 97004, Taiwan
3Department of Computer Science & Information Engineering, Tamkang University, New Taipei City 25137, Taiwan
4Bioinformatics Core Laboratory, Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan
5Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien 97004, Taiwan

Received 11 April 2014; Revised 14 August 2014; Accepted 16 August 2014; Published 16 September 2014

Academic Editor: Tzong-Yi Lee

Copyright © 2014 Kuei-Fang Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Various biological effects are associated with radiation exposure. Irradiated cells may elevate the risk for genetic instability, mutation, and cancer under low levels of radiation exposure, in addition to being able to extend the postradiation side effects in normal tissues. Radiation-induced bystander effect (RIBE) is the focus of rigorous research as it may promote the development of cancer even at low radiation doses. Alterations in the DNA sequence could not explain these biological effects of radiation and it is thought that epigenetics factors may be involved. Indeed, some microRNAs (or miRNAs) have been found to correlate radiation-induced damages and may be potential biomarkers for the various biological effects caused by different levels of radiation exposure. However, the regulatory role that miRNA plays in this aspect remains elusive. In this study, we profiled the expression changes in miRNA under fractionated radiation exposure in human peripheral blood mononuclear cells. By utilizing publicly available microRNA knowledge bases and performing cross validations with our previous gene expression profiling under the same radiation condition, we identified various miRNA-gene interactions specific to different doses of radiation treatment, providing new insights for the molecular underpinnings of radiation injury.