A Multiplex Snapback Primer System for the Enrichment and Detection of JAK2 V617F and MPL W515L/K Mutations in Philadelphia-Negative Myeloproliferative Neoplasms

Figure 3

ARMS for JAK2 V617F mutation. Amplified DNA products from the ARMS assay were subjected to 2% agarose electrophoresis for mutation identification. The 469 bp product served as the amplification control. Bands of 229 bp suggested the presence of the wild-type allele, while the mutant allele was indicated by the bands of 279 bp. M: 1,000 bp DNA ladder; BLK: no template amplification control; 1: HEL cell line DNA; 2: RPMI 8226 cell line DNA; 3: patient sample identified as JAK2 V617F-homozyous positive; 4: patient sample with heterozygous JAK2 V617F mutation.