Modulation of Steroidogenic Pathway in Rat Granulosa Cells with Subclinical Cd Exposure and Insulin Resistance: An Impact on Female Fertility

Table 2

Details of genes, accession number, primers, annealing Tm, and expected size of the PCR-amplified c-DNA.

Gene name	GenBank accession number	Sequence of the primer; Fw primer; Rv primer	Annealing temperature (°C)	Product size (bp)
StAR	NM_ 31558	5′ AGGCAGGGGGATCTTTCTAA 3′; 5′ TGCCTGACTAGGGTTTCGTT 3′	56.8	330
17β-HSD	AF035156	5′ CCTCCTTCGCCACTATCAGC 3′; 5′ GGAGACAAATGAGGGCTC 3′	55.4	653
CYP19A1	NM_017085	5′ GGAATCCATCAAGCAGCATT 3′; 5′ TTCCACCTCCGGATACTCTG 3′	58	493
β-Actin	V01217	5′ CCTGCTTGCTGATCCACA 3′; 5′ CTGACCGAGCGTGGCTAC 3′	57	505