miRSeq: A User-Friendly Standalone Toolkit for Sequencing Quality Evaluation and miRNA Profiling
Figure 3
Illustration of readMap alignment output. From the output results of readMap, we may compare read category, 3′ end modification, and isomiR patterns among libraries. (a) readMap reports the read categories between libraries. High percentage of rRNA reads in L1 library resulted from the nonstandard protocol in the in-gel size fraction procedure. The non-miRNA reads accounted for much higher proportions in the libraries with less enriched read length at 22-nt. (b) readMap reports the 3′ modification patterns of miRNA reads. Such information is consistent between the libraries from the same species. Here, only the patterns more frequent than 1% are shown. (c) The top sequence denotes pre-miRNA sequence with mature miRNA marked in upper case. All isomiR forms are shown according to their relative position in pre-miRNA. The 3′ end modification patterns are presented in lower case. The middle digits and right-hand side information containing comma denote the read count and position shift of each isomiR.
