Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 463123, 6 pages
http://dx.doi.org/10.1155/2014/463123
Research Article

Adenylyl Cyclase Signaling in the Developing Chick Heart: The Deranging Effect of Antiarrhythmic Drugs

1Department of Physiology, Faculty of Science, Charles University in Prague, 128 43 Prague, Czech Republic
2Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic
3Institute of Anatomy, First Faculty of Medicine, Charles University in Prague, 128 00 Prague, Czech Republic

Received 28 February 2014; Revised 14 May 2014; Accepted 29 May 2014; Published 23 June 2014

Academic Editor: Diego Franco

Copyright © 2014 Lucie Hejnova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The adenylyl cyclase (AC) signaling system plays a crucial role in the regulation of cardiac contractility. Here we analyzed the key components of myocardial AC signaling in the developing chick embryo and assessed the impact of selected β-blocking agents on this system. Application of metoprolol and carvedilol, two commonly used β-blockers, at embryonic day (ED) 8 significantly downregulated (by about 40%) expression levels of AC5, the dominant cardiac AC isoform, and the amount of Gsα protein at ED9. Activity of AC stimulated by forskolin was also significantly reduced under these conditions. Interestingly, when administered at ED4, these drugs did not produce such profound changes in the myocardial AC signaling system, except for markedly increased expression of Giα protein. These data indicate that β-blocking agents can strongly derange AC signaling during the first half of embryonic heart development.