Punicalagin and Ellagic Acid Demonstrate Antimutagenic Activity and Inhibition of Benzo[a]pyrene Induced DNA Adducts

Table 7

Effect of ellagic acid on the sodium azide induced mutagenicity in Salmonella typhimurium.

Treatment	Dose (μM)	Number of His^+ revertants colonies/plate (mean ± SE)
		TA 97a	TA 98	TA 100	TA 102
Spontaneous		142.0±5.3	33.7±2.0	128.3±4.9	240.7±12.1
Positive control (NaN3)	1.5 μg	256.0±11.7	52.3±1.8	361.3±11.0	355.0±15.5
^aEllagic acid	50	189.7±6.4	50.7±1.5	179.7±0.1	310.7±27.0
	100	163.0±5.2	38.0±1.7	170.0±5.7	288.0±21.0
	250	145.3±4.3	34.3±2.4	155.7±10.3	262.3±13.2
	500	134.0±3.8	30.0±1.2	132.3±8.6	248.0±19.1
^bEllagic acid + NaN3	50	242.0±11.8 (21.1)	52.0±1.7 (20.0)	340.7±9.4 (11.4)	345.3±26.7 (21.9)
	100	219.3±7.1 (39.4)	48.3±2.7 (27.9)	312.3±8.4^∗ (25.6)	328.0±28.2 (40.3)
	250	196.7±8.2^∗ (53.6)	43.3±2.6^∗ (50.0)	266.0±7.8^∗∗ (46.4)	308.0±23.6^∗ (50.7)
	500	168.0±7.0^∗∗ (72.1)	38.0±1.7^∗∗ (64.2)	210.3±12.4^∗∗∗ (65.9)	288.3±19.2^∗∗ (62.3)
𝑅^2		0.99	0.98	0.99	0.97

aNegative control; ^bpreincubation test; values in parenthesis are % inhibition of mutagenicity.
^∗𝑃<0.05; ^∗∗𝑃<0.005 and ^∗∗∗𝑃<0.001; NaN₃: sodium azide; 𝑅²: linear regression analysis.