Research Article

Induction of Apoptosis of 2,4′,6-Trihydroxybenzophenone in HT-29 Colon Carcinoma Cell Line

Figure 4

(a) Cells were treated with compound (30 µM) for 24 h, 48 h, and 72 h which induced morphological changes typical of apoptosis in HT-29 colon carcinoma cells. Control or treated cells were observed under inverted microscope and photographed. (b) Control or treated cells were observed under phase contrast microscope and photographed. (c) Treatment with IC50 value (30 µM) of 2,4′,6-trihydroxy-4-methoxybenzophenone for 48 h induces morphological changes typical of apoptosis in HT-29 colon cancer cells. After being stained with acridine orange and propidium iodide, treated cells were observed under fluorescence microscopy—live cells stained (green colour), apoptotic cells (orange), and necrotic cells or dead cells (red).
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