Induction of Apoptosis of 2,4′,6-Trihydroxybenzophenone in HT-29 Colon Carcinoma Cell Line
Figure 5
(a) Effects of 2,4′,6-trihydroxy-4-methoxybenzophenone on induction of apoptosis in HT-29 cells. The cells were treated with different concentrations of compound (25 µM, 50 µM, and 75 µM) in a time-dependent manner (24 h, 48 h, and 72 h), labelled with FITC annexin V and PI. Viable cells = LL; Early apoptotic cells = LR; late or secondary necrotic cells = UR; primary necrotic cells = UL. (b) Histogram representation of the quantitative percentage of viable cells (LL), early apoptotic cells (LR), primary necrotic cells (UL), and late apoptotic cells or secondary necrotic cells (UR) of HT-29 treatment with different concentration of 2,4′,6-trihydroxy-4-methoxybenzophenone for 24 h, 48 h, and 72 h.
