Research Article
Genetic Analysis of Diversity within a Chinese Local Sugarcane Germplasm Based on Start Codon Targeted Polymorphism
Table 2
Nucleotide sequence and amplification efficiency of 20 sugarcane SCoT primers.
| Number | Primer | Sequence (5′-3′) | GC (%) | NTB | NPB | PPB | PIC |
| 1 | P1 | CAACAATGGCTACCACCA | 50 | 11 | 10 | 90.91 | 0.895 | 2 | P3 | CAACAATGGCTACCACCG | 56 | 10 | 8 | 80.00 | 0.892 | 3 | P6 | CAACAATGGCTACCACGC | 56 | 9 | 9 | 100.0 | 0.884 | 4 | P8 | CAACAATGGCTACCACGT | 50 | 8 | 8 | 100.0 | 0.842 | 5 | P11 | AAGCAATGGCTACCACCA | 50 | 10 | 9 | 90.00 | 0.887 | 6 | P12 | ACGACATGGCGACCAACG | 61 | 9 | 8 | 88.89 | 0.864 | 7 | P15 | ACGACATGGCGACCGCGA | 67 | 8 | 7 | 87.50 | 0.854 | 8 | P17 | ACCATGGCTACCACCGAG | 61 | 9 | 9 | 100.0 | 0.875 | 9 | P22 | AACCATGGCTACCACCAC | 56 | 8 | 7 | 87.50 | 0.867 | 10 | P23 | CACCATGGCTACCACCAG | 61 | 9 | 9 | 100.0 | 0.886 | 11 | P25 | ACCATGGCTACCACCGGG | 67 | 9 | 8 | 88.89 | 0.834 | 12 | P26 | ACCATGGCTACCACCGTC | 61 | 7 | 6 | 85.71 | 0.840 | 13 | P27 | ACCATGGCTACCACCGTG | 61 | 9 | 9 | 100.0 | 0.884 | 14 | P28 | CCATGGCTACCACCGCCA | 67 | 5 | 5 | 100.0 | 0.790 | 15 | P29 | CCATGGCTACCACCGGCC | 72 | 11 | 10 | 90.91 | 0.892 | 16 | P31 | CCATGGCTACCACCGCCT | 67 | 11 | 10 | 90.91 | 0.907 | 17 | P32 | CCATGGCTACCACCGCAC | 67 | 6 | 6 | 100.0 | 0.806 | 18 | P35 | CATGGCTACCACCGGCCC | 72 | 7 | 6 | 85.71 | 0.783 | 19 | P37 | ACGACATGGCGACCAGCG | 66 | 10 | 9 | 90.00 | 0.859 | 20 | P39 | AACCATGGCTACCACCGC | 61 | 10 | 10 | 100.0 | 0.882 | | Subtotal | | | 176 | 163 | | | | Average | | | | | 92.85 | 0.861 |
|
|
Notes: NTB: number of total bands; NPB: number of polymorphic bands; PPB: percentage of polymorphic bands; PIC: polymorphism information content.
|