Molecular Characterization of a Recombinant Manganese Superoxide Dismutase from Lactococcus lactis M4
Figure 5
IEF PAGE analysis and SOD activity staining of purified SOD. Each lane contained 1 μg of purified SOD. Lane 1: broad range pI marker; lane 2: purified SOD with pI value of 4.5; lane 3: purified SOD without any treatment as control; lane 4: SOD treated with 2 mM KCN; lane 5: SOD treated with 3 mM H2O2; and lane 6: SOD treated with 2 mM KCN and 3 mM H2O2. The achromatic zones of the control and the treated enzyme showed no significant differences.
