Lung Cancer Stem Cell Lose Their Stemness Default State after Exposure to Microgravity

Figure 2

(a) A representative experiment of DNA content analysis by flow cytometry. (b) Quantitative analysis of DNA content after 24 h on ground or RPM exposure showing that RPM evokes shift from G0/G1 phase (Student’s -test, ), into the S phase (Student’s -test, ). The data shown represent the mean ± SD (). (c) A representative WB analysis of cyclin D1 and cyclin B1 in H460 spheroids exposed or not to RPM. Vinculin was used as loading control. (d) Apoptosis of H460 spheroids growing on ground and in simulated gravity. Graphs are representative of three independent experiments. (e) Apoptosis rate in CSCs growing on ground and in RPM-condition. Data represent mean ± SD (Student’s -test, ).