Research Article

The Putative HORMA Domain Protein Atg101 Dimerizes and Is Required for Starvation-Induced and Selective Autophagy in Drosophila

Figure 4

Ultrastructural analysis of Atg101[KK+HMS] RNAi cells. A fat body lobe containing a single GFP-marked RNAi cell (a) was embedded in plastic, and the same cell is highlighted in semithin (b) and low-magnification ultrastructural images (c). High magnification images show autophagosomes (short arrows) and autolysosomes (long arrows) in control cells ((d), and also in (e), bottom). The generation of such autophagic structures is inhibited in Atg101 RNAi cells ((e), top, and (f)). Asterisk marks a potential cytoplasmic protein aggregate, which can be recognized by its homogenous appearance and exclusion of organelles and ribosomes.
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