The Putative HORMA Domain Protein Atg101 Dimerizes and Is Required for Starvation-Induced and Selective Autophagy in Drosophila

Figure 9

Comparison of the Prosite HORMA domain logo with the sequence alignment derived from the multiple structure alignment of the predicted Atg101 structure, Mad2 (2V64, chain A), and Atg13 (4J2G, chain A). Residues in Drosopohila Atg101 corresponding to the most frequent one in the Prosite logo and mostly conserved in other Atg101 proteins are highlighted with an orange background. Residues with a similar character to those in the logo and conserved in Atg101 proteins are highlighted with cyan background. Dots denote gaps arisen in the original structural alignment, dashes represent gaps relative to the Prosite logo. Note that the alignment is adjusted to match the full Prosite logo, so insertions relative to that have been removed from all sequences, and thus they do not correspond to the full native ones. In the Prosite logo, positively and negatively charged residues are colored blue and red, respectively; those with hydrophobic side chains are shown in black; Gln and Asn in magenta; other amino acids with hydrophilic side chains and glycine in green.