Preparation and Characterization of Gelatin-Based Mucoadhesive Nanocomposites as Intravesical Gene Delivery Scaffolds

Figure 5

Fluorescence images of AY-27 cells at day 10 after transfecting with hydrogel entrapped LV-GFP. AY-27 rat bladder cancer cells (a) were treated with (b) hydrogels (H), (c) lentivirus (LV), (d) hydrogels containing lentivirus (H-LV), (e) LV constructed with the GFP gene (LV-GFP), and (f) hydrogels containing LV-GFP (H-LV-GFP). Cells expressing GFP appear green while cell nuclei stained with DAPI appear blue (magnification ×200).

(a)

(b)

(c)

(d)

(e)

(f)