[Retracted] Leaf Rolling and Stem Fasciation in Grass Pea (Lathyrus sativus L.) Mutant Are Mediated through Glutathione-Dependent Cellular and Metabolic Changes and Associated with a Metabolic Diversion through Cysteine during Phenotypic Reversal

Table 3

Activities of γ-glutamylcysteine synthetase (γ-ECS, nmol min⁻¹ g⁻¹ FW), serine acetyl transferase (SAT, U mg⁻¹ protein), O-acetylserine thiol lyase (OAS-TL, nmol cysteine min⁻¹ mg⁻¹ protein), superoxide dismutase (SOD, U min⁻¹ mg⁻¹ protein), ascorbate peroxidase (APX, µmol ascorbate oxidized min⁻¹ mg⁻¹ protein), glutathione reductase (GR, nmol NADPH oxidized min⁻¹ mg⁻¹ protein), glutathione-S transferases (GSTs, U mg⁻¹ protein), and glutathione peroxidase (GPX, nmol NADPH min⁻¹ mg⁻¹ (protein)), L-cysteine desulfhydrase (LCD, nmol H₂S min⁻¹ mg⁻¹ protein), D-cysteine desulfhydrase (DCD, nmol H₂S min⁻¹ mg⁻¹ protein), hydrogen sulfide level (H₂S, µmol g⁻¹ FW) and glycolate oxidase (GO, µmol glyoxylate min⁻¹ mg⁻¹ protein) H₂O₂ (µmol g⁻¹ FW), malondialdehyde (MDA, nmol g⁻¹ FW) in rlfL-1 mutant and its mother variety Biol-212 under untreated and BSO-treated conditions.

Traits	MC	MuC	MuT	TM
γ-ECS	6.8±1.1^b	23.8±4.2^a	ND	ND
SAT	0.65±0.08^a	0.69±0.05^a	0.64±0.03^a	0.64±0.07^a
OAS-TL	23.6±1.97^b	70.2±3.33^a	69.7±3.12^a	22.5±1.90^b
SOD-leaf	180.3±9.1^b	178.9±6.3^b	359.7±10.5^a	356.6±10.6^a
SOD-stems	165.3±7.3^b	170.7±5.9^b	343.4±9.5^a	350.8±11.0^a
APX-leaf	131.3±8.8^b	328.3±11.2^a	34.56±2.4^a	35.66±1.9^a
APX-stems	111.5±6.7^b	282.3±11.2^a	29.67±2.8^c	30.59±2.5^c
GR-leaf	31.2±2.2^b	131.04±12.5^a	129.8±12.3^a	30.9±2.1^b
GR-stems	33.2±2.2^b	134.07±10.8^a	130.9±12.9^a	33.9±2.8^b
GSTs-leaf	0.22±0.08^b	1.21±2.8^a	0.30±0.08^b	0.05±0.008^c
GSTs-stem	0.25±0.10^b	1.25±2.9^a	0.33±0.07^b	0.06±0.008^c
GPX-leaf	7.1±0.98^b	13.49±3.3^a	8.99±0.89^b	4.72±0.77^c
GPX-stems	7.8±0.99^b	12.87±3.2^a	9.01±0.90^b	4.69±0.69^c
LCD-leaf	20.38±0.64	11.67±0.45	46.67±0.78	19.38±0.63
LCD-stems	17.54±0.45^b	10.58±0.39^c	37.03±0.41^a	18.54±0.41^b
DCD-leaf	16.15±0.63^b	9.87±0.37^c	40.23±0.69^a	16.03±0.59^b
DCD-stems	13.18±0.55	7.99±0.29	28.01±0.72	13.20±0.51
H2S level-leaf	0.047±0.003^b	0.029±0.002^c	0.087±0.006^a	0.050±0.003^b
H2S level-stems	0.033±0.002^b	0.025±0.001^c	0.088±0.007^a	0.034±0.002^b
GO activity-leaf	0.85±0.03^b	0.76±0.03^b	2.28±0.09^a	0.83±0.02^b
H2O2-leaf	3.32±0.81^c	3.19±0.62^c	21.67±0.98^a	14.56±0.34^b
H2O2-stem	3.21±0.67^c	3.05±0.62^c	17.13±0.54^a	10.44±0.29^b
MDA-leaf	4.23±0.71^c	4.13±0.65^c	28.78±0.87^a	16.23±0.33^b
MDA-stems	3.99±0.53^c	3.20±0.44^c	22.56±0.49^a	14.31±0.29^b
EL%-leaf	4.04±0.38^c	4.16±0.38^c	27.34±0.55^a	12.87±0.22^b
EL%-stems	3.87±0.29^c	4.10±0.35^c	25.58 ± 0.50a	12.32±0.19^b

Data are means ± SE of at least four replicates. Means followed by same alphabets are not significantly (𝑃 > 0.05) different at ANOVA followed by Duncan’s multiple range test. MC-untreated (no BSO) mother control, MuC-untreated (no BSO), MuT-BSO-treated mutant, and TM-BSO treated mother plant.