Methylamine-Sensitive Amperometric Biosensor Based on (His)6-Tagged Hansenula polymorpha Methylamine Oxidase Immobilized on the Gold Nanoparticles
Figure 4
Characteristics of the developed MA biosensor under applied potential of −50 mV at 22°C. The highest current response was chosen as 100% in all experiments. Selectivity test was performed with 0.1 mM solutions of MA, DMA, EA, and TMA in 20 mM PB, pH 7.5 (a); storage stability was checked using 0.05 mM MA in 20 mM PB, pH 7.5, at room temperature during 16 days (b). The bioelectrode was kept at +4°C in 20 mM PB, pH 7.5.
