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BioMed Research International
Volume 2014, Article ID 483596, 10 pages
Research Article

Quantitative Evaluation of E1 Endoglucanase Recovery from Tobacco Leaves Using the Vacuum Infiltration-Centrifugation Method

Department of Chemical Engineering and Materials Science, University of California at Davis, 1 Shields Avenue, Davis, CA 95616, USA

Received 28 November 2013; Revised 4 April 2014; Accepted 29 April 2014; Published 26 May 2014

Academic Editor: Qiang “Shawn” Chen

Copyright © 2014 Nathaniel J. Kingsbury and Karen A. McDonald. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Included in the supplementary material is mean data for each extract (the WHE, the UHE, and the AWF and the RF) from the experiments recovering peroxidase (Supplementary Table 1), E1cd (Supplementary Table 3), and E1holo (Supplementary Table 5). Also provided are tables for these respective experiments with the mean values for the POI percent yield, purity, and concentration of each extract.

The supplementary material also includes a figure showing the change in weight of the leaf tissue in the indigo carmine and peroxidase experiments during the course of three rounds of VI-C. The weight of the leaf tissue increases and decreases as fluid is moved into and out of its intercellular space.

  1. Supplementary Material