Freeze-Drying of Plant Tissue Containing HBV Surface Antigen for the Oral Vaccine against Hepatitis B

Figure 2

Western blot analysis of S-HBsAg in freeze-dried tissue using the specific polyclonal antibody. Lanes: M—protein molecular weight marker (MBI Fermentas), P—S-HBsAg positive control (Meridian Life Sciences). “S” and “N” analysed freeze-dried transgenic lettuce containing S-HBsAg and nontransgenic plant (negative control), respectively, soaked with a given excipient, p48—S-HBsAg dimer.