Freeze-Drying of Plant Tissue Containing HBV Surface Antigen for the Oral Vaccine against Hepatitis B

Figure 7

The effect of storage temperature on stability of S-HBsAg VLPs and total antigen in lyophilised tissue. Freeze-drying was performed according to the 20°C/22 h–22°C/2 h profile, with 500 mM sucrose as the protective excipient. Letter indexes mark statistically homogenous groups, separately for VLP-assembled S-HBsAg (small caps) and the total antigen (capitals).