MicroRNA Profiling Reveals Unique miRNA Signatures in IGF-1 Treated Embryonic Striatal Stem Cell Fate Decisions in Striatal Neurogenesis In Vitro

Figure 2

Lineage commitment and self-renewal by IGF-derived ESSCs at 20 DIV—Differentiated phenotypes of ESSCs were characterized on 20th DIV using several stage specific markers. Immunoflouroscence micrographs displayed appearance of self-renewing ESSCs by SOX-2 and nestin expressing neurospheres. Representative confocal images depict generation of oligodendrocytes, astrocytes and neurons as O1+ cells, GFAP+ cells, and MAP-2+ cells respectively in ESSCs derived from different treatment groups (A to E). Nuclei were stained by acridine orange counter staining. Bar = 100 m.