Single Amino Acid Arginine Deprivation Triggers Prosurvival Autophagic Response in Ovarian Carcinoma SKOV3
Figure 6
Transcriptional silencing of BECLIN 1 expression leads to decreased viability of SKOV3 cells under arginine starvation. (a) Western blot analysis of BECLIN 1 protein in SKOV3 cells transfected with BECLIN 1 siRNA or with nonsilencing control siRNA. β-Actin served as the loading standard. Densitometry quantification and the calculation of the relative band intensity were performed as described in Section 2. Intensity represents the mean value of three independent experiments and is expressed as means ± SD; (b) histogram represents the effect of BECLIN 1 silencing on the viability of arginine-starved cells and their proliferative potential after arginine resupplementation. .
