Expression of Mesenchymal Stem Cells-Related Genes and Plasticity of Aspirated Follicular Cells Obtained from Infertile Women
Figure 3
Epithelial-like phenotype of aspirated follicular cells (AFCs) in culture dish immediately after enrichment with hypoosmotic method (a). Fibroblast-like phenotype of AFCs in culture dish 48 hours after isolation (b). AFCs positive for alkaline phosphatase activity (pink-violet) (c). Differentiation of AFCs into osteogenic-like cells, von Kossa-positive staining (d). Differentiation of AFCs into adipogenic-like cells, accumulation of lipid droplets (dark red) (e). Differentiation of AFCs into pancreatic-like cells, dithizone-positive (bright red-pink) cell cluster (f). Scale bar: 100 μm (a, b, c, e) and 50 μm (d, f).
