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BioMed Research International
Volume 2014 (2014), Article ID 510408, 8 pages
Research Article

Establishment of Cell Lines from Both Myeloma Bone Marrow and Plasmacytoma: SNU_MM1393_BM and SNU_MM1393_SC from a Single Patient

1Department of Internal Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-Ku, Seoul 110-744, Republic of Korea
2Cancer Research Institute, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-Ku, Seoul 110-799, Republic of Korea
3Functional Genome Institute, PDXen Biosystem Inc., 437 Dongil-ro, Kwangjin-Ku, Seoul 143-901, Republic of Korea

Received 15 April 2014; Accepted 30 June 2014; Published 12 August 2014

Academic Editor: Dong Soon Lee

Copyright © 2014 Youngil Koh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. We tried to establish clinically relevant human myeloma cell lines that can contribute to the understanding of multiple myeloma (MM). Materials and Methods. Mononuclear cells obtained from MM patient’s bone marrow were injected via tail vein in an NRG/SCID mouse. Fourteen weeks after the injection, tumor developed at subcutis of the mouse. The engraftment of MM cells into mouse bone marrow (BM) was also observed. We separated and cultured cells from subcutis and BM. Results. After the separation and culture of cells from subcutis and BM, we established two cell lines originating from a single patient (SNU_MM1393_BM and SNU_MM1393_SC). Karyotype of the two newly established MM cell lines showed tetraploidy which is different from the karyotype of the patient (diploidy) indicating clonal evolution. In contrast to SNU_MM1393_BM, cell proliferation of SNU_MM1393_SC was IL-6 independent. SNU_MM1393_BM and SNU_MM1393_SC showed high degree of resistance against bortezomib compared to U266 cell line. SNU_MM1393_BM had the greater lethality compared to SNU_MM1393_SC. Conclusion. Two cell lines harboring different site tropisms established from a single patient showed differences in cytokine response and lethality. Our newly established cell lines could be used as a tool to understand the biology of multiple myeloma.