Review Article

Significance of Chromosome 9p Status in Renal Cell Carcinoma: A Systematic Review and Quality of the Reported Studies

Table 3

Prevalence of chromosome 9/9p deletion according to technique and candidate locus or gene studied.

StudyGenetic techniqueThreshold for deletionCandidate locus/geneNumber of 9p deleted cases (%)

(1) Antonelli et al., 2010 [31]Karyotyping20 metaphases*Chromosome 9/9p19/131 (14.5%)

(2) Brunelli et al., 2008 [32]FISHMean + 4SD the percentage of abnormal nuclei in normal tissue → 30% for monosomy and 31% for LOHLSI p16 (INK4A,B, ARF)13/73 (18%)

(3) Gunawan et al., 2003 [33]Karyotyping20 metaphases*Chromosome 9/9p8/50 (16%)

(4) Klatte et al., 2009 [34]Karyotyping20 metaphases*Chromosome 9/9p6/65 (9%)

(5) La Rochelle et al., 2010 [29]FISHIf majority of nuclei were abnormalLSI p16 (INK4A,B, and ARF)44/316 (13.9%)
Karyotyping20 metaphases*Chromosome 9/9p53/388 (13.6%)

(6) Li et al., 2011 [35]Microsatellite analysisThe signal from one allele was >50% reduced in tumour DNA compared with normal control
The appearance of new bands defined as instability (MSI)
D9S168 (9p23–9p24.2)
D9S171 (9p21)
D9S157 (9p22)
D9S1749 (9p21)
34/92 (36.9%)
4/29 (13.7%)
3/23 (13%)
1/27 (3.7%)

(7) Moch et al., 1996 [36]CGHMean green : red ratio ±1 SDChromosome 9/9p loss10/41 (24%)

(8) Presti et al., 2002 [37]Microsatellite analysisThe signal from one allele was >50% reduced in tumour DNA compared with normal controlD9S925 (9p22)
D9S921 (9p22)
21/67 (31%)

(9) Sanjmyatav et al., 2011 [38]Array CGHMean green : red ratio ±1.5 SD9p21.3 p24.111/53 (21%)
FISHMean − 2SD the percentage of normal diploid cells per 10 normal tissues → 10%LSI p16 (9p21)Described as 89% sensitivity

(10) Schraml et al., 2000 [40] Microsatellite analysisThe signal from one allele was >50% reduced in tumour DNA compared with normal controlD9S970 (9p12-9p13)2/25 (8%)
D9S171 (9p13)6/29 (21%)
D9S1748 (9p21)2/32 (6%)
D9S156 (9p21)1/25 (4%)
Allelic deletion in 9p with at least one microsatellite →8/37 (22%)

(11) Schraml et al., 2001 [39] Microsatellite analysisThe signal from one allele was >50% reduced in tumour DNA compared with normal controlD9S970 (9p12-9p13)5/68 (7%)
D9S171 (9p13)12/59 (20%)
D9S1748 (9p21)7/73 (10%)
D9S156 (9p21)8/68 (9%)
Allelic deletion in 9p with at least one microsatellite → 21/88 (24%)
FISH40% monosomy and 50% LOH CDKN2A6/54 (11%)
Sequence analysisCDKN2A → 24 bp deletion within exon 113/113 (12%)

LSI: locus specific identifier.
*Analysis in accordance with the International Standing Committee on Human Cytogenetic Nomenclature.