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Study | Genetic technique | Threshold for deletion | Candidate locus/gene | Number of 9p deleted cases (%) |
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(1) Antonelli et al., 2010 [31] | Karyotyping | 20 metaphases* | Chromosome 9/9p | 19/131 (14.5%) |
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(2) Brunelli et al., 2008 [32] | FISH | Mean + 4SD the percentage of abnormal nuclei in normal tissue → 30% for monosomy and 31% for LOH | LSI p16 (INK4A,B, ARF) | 13/73 (18%) |
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(3) Gunawan et al., 2003 [33] | Karyotyping | 20 metaphases* | Chromosome 9/9p | 8/50 (16%) |
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(4) Klatte et al., 2009 [34] | Karyotyping | 20 metaphases* | Chromosome 9/9p | 6/65 (9%) |
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(5) La Rochelle et al., 2010 [29] | FISH | If majority of nuclei were abnormal | LSI p16 (INK4A,B, and ARF) | 44/316 (13.9%) |
Karyotyping | 20 metaphases* | Chromosome 9/9p | 53/388 (13.6%) |
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(6) Li et al., 2011 [35] | Microsatellite analysis | The signal from one allele was >50% reduced in tumour DNA compared with normal control The appearance of new bands defined as instability (MSI) | D9S168 (9p23–9p24.2) D9S171 (9p21) D9S157 (9p22) D9S1749 (9p21) | 34/92 (36.9%) 4/29 (13.7%) 3/23 (13%) 1/27 (3.7%) |
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(7) Moch et al., 1996 [36] | CGH | Mean green : red ratio ±1 SD | Chromosome 9/9p loss | 10/41 (24%) |
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(8) Presti et al., 2002 [37] | Microsatellite analysis | The signal from one allele was >50% reduced in tumour DNA compared with normal control | D9S925 (9p22) D9S921 (9p22) | 21/67 (31%)
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(9) Sanjmyatav et al., 2011 [38] | Array CGH | Mean green : red ratio ±1.5 SD | 9p21.3 p24.1 | 11/53 (21%) |
FISH | Mean − 2SD the percentage of normal diploid cells per 10 normal tissues → 10% | LSI p16 (9p21) | Described as 89% sensitivity |
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(10) Schraml et al., 2000 [40] |
Microsatellite analysis | The signal from one allele was >50% reduced in tumour DNA compared with normal control | D9S970 (9p12-9p13) | 2/25 (8%) |
D9S171 (9p13) | 6/29 (21%) |
D9S1748 (9p21) | 2/32 (6%) |
D9S156 (9p21) | 1/25 (4%) |
Allelic deletion in 9p with at least one microsatellite → | 8/37 (22%) |
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(11) Schraml et al., 2001 [39] |
Microsatellite analysis | The signal from one allele was >50% reduced in tumour DNA compared with normal control | D9S970 (9p12-9p13) | 5/68 (7%) |
D9S171 (9p13) | 12/59 (20%) |
| | D9S1748 (9p21) | 7/73 (10%) |
| | D9S156 (9p21) | 8/68 (9%) |
| | Allelic deletion in 9p with at least one microsatellite → | 21/88 (24%) |
FISH | 40% monosomy and 50% LOH | CDKN2A | 6/54 (11%) |
Sequence analysis | | CDKN2A → 24 bp deletion within exon 1 | 13/113 (12%) |
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