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Figure 4: Immunohistochemical staining with α-smooth muscle actin for quantification of the blood vessels in myocardium in the AMI area of the control (a), AMI area of the tac-treated (b), peri-AMI area of the control (c), peri-AMI area of the tac-treated (d), and normal groups (e). Note the significantly more small vessels (≤25  m) (arrows) in normal than in the controls and the tac-treated minipigs (∗  versus other groups, all ), and significantly reduced small vessels in the controls than in the tac-treated minipigs († versus ‡, versus , all ) (f). Scale bars in right lower corner represent 20  m.