Research Article

Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus

Table 3

Detection of H7N9 influenza viruses in clinical samples by virus isolation, RT-LAMP, and RT-PCR.

Source of samplesaTotal number of samplesH7N9 virus positive samples
Virus isolationRT-LAMPRT-PCR

Chickens 142101010
Pigeons33333
Ducks30000
Environmentb54553

Total 259181816

All samples were collected from poultry farms and live poultry markets in Shanghai and Anhui Province.
bThe samples collected from the environment included soil, water, and fecal samples from different areas of the markets.