BioMed Research International / 2014 / Article / Fig 5

Research Article

Mesenchymal Stem Cell-Induced Doxorubicin Resistance in Triple Negative Breast Cancer

Figure 5

IL-8 induced BCRP protein expression in MDA-MB-231 cells. (a) Human recombinant IL-8 dose-dependently elevated BCRP protein expression after 24 hours of examination by Western blotting. (b) IL-8 neutralizing antibody (5 μg/mL) antagonized MSC-ad conditioned medium-induced BCRP protein expression. IgG isotype control antibody was used as negative control. Graphs showed mean ± SEM of three independent experiments. to control group; to CM-treated group. CM, conditioned medium from adipose-derived mesenchymal stem cells (MSC-ad); NeuAb, IL-8 neutralizing antibody.

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