Target Capture and Massive Sequencing of Genes Transcribed in Mytilus galloprovincialis

Table 2

Sequencing output data and summary of de novo assembling results.

Sequencing output	RUN_1		RUN_2		RUN_1 + 2
Total reads	472,122		473,409		945,531
Total high quality reads	287,362		339,407		626,769
Average length (bp)	380		114		235
On-target reads (number and %)	179,201	62%	175,432	52%	354,633	57%
Covered targets (number and %)	1,262	83%	1,032	68%	1,355	89%
De novo assembly	On-target reads		Off-target reads		All dataset
Total contigs	5,547		12,423		14,339
Total assembled reads (number and %)	279,922	79%	347,439	45%	444,145	71%
Average contig length (bp)	490		476		511
N50 (bp)	557		523		552
N75 (bp)	388		402		405
Longest contig (bp)	2,234		3,538		4,217
Contigs with blast annotation	28%		21%		44%

Total raw and HQ reads, average length (bp), number of mapped reads (on-target), and covered contigs are reported for the subsets (RUN_1, RUN_2) and total sequenced data (RUN_1 + 2).
De novo assembly of the reads on-target and off-target and of the whole dataset. Number of resulting contigs and related reads, contig length, quality parameters, longest contig, and percentage of annotated contigs are reported.