Apoptosis Induction by Polygonum minus Is Related to Antioxidant Capacity, Alterations in Expression of Apoptotic-Related Genes, and S-Phase Cell Cycle Arrest in HepG2 Cell Line

Table 2

Antioxidant activities of fractions isolated from EtOAc crude extract of P. minus.

EtOAc fractions	TPC; (mg GAE/g extract)	DPPH scavenging activity (EC50) (μg/mL)	FRAP value μmol Fe (II)/mg dry weight extract)
F1	ND	>1 mg/mL	ND
F2	14.2 ± 3.5^a	568 ± 8.7^a	147.53 ± 8.4^a
F3	16.3 ± 4.2^a	348.6 ± 5.7^b	187.46 ± 10.7^b
F4	21.7 ± 3.1^b	200.2 ± 2.1^c	224.41 ± 26.5^c
F5	57.2 ± 6.8^c	140.3 ± 3.2^d	270.76 ± 17.2^d
F6	96 ± 6.7^d	90.2 ± 4.5^e	356.84 ± 10.9^e
F7	113.16 ± 6.2^e	30.5 ± 3.2^f	1169.1 ± 20.3^f
Trolox	99 ± 2.4^d	ND	ND
Chlorogenic acid	ND	19.95 ± 2.8^g	ND
Ascorbic acid	ND	ND	3305.67 ± 51.5^g

Values are mean ± S.D (𝑛=3). Values with different alphabets in the same column are significantly different (P < 0.05, ANOVA and Bonferroni test). GAE: gallic acid equivalents. ND: not determined.