Apoptosis Induction by Polygonum minus Is Related to Antioxidant Capacity, Alterations in Expression of Apoptotic-Related Genes, and S-Phase Cell Cycle Arrest in HepG2 Cell Line

Table 5

IC₅₀ values of different fractions from EtOAc extract of P. minus on HepG2 cell line.

Fractions	IC50 (μg/mL)
F1	ND
F2	52.86 ± 2.4^a
F3	63.19 ± 2.64^b
F4	46.12 ± 6.1^a
F5	65.96 ± 2.5^b
F6	42.08 ± 3.22^a
F7	25.75 ± 1.5^c

HepG2 cells (2 × 10⁶/well) were incubated with P. minus fractions for 72 h at 37°C. Results are mean ± S.D. (𝑛=3). Values with different alphabets are significantly different (P < 0.05, ANOVA and Bonferroni test). ND: not determined.