Virulence Factors Associated with Pediatric Shigellosis in Brazilian Amazon

Table 1

The striking points employed for the detection of virulence markers of Shigella.

Gene	Amplicon size (bp)	Primer	Annealing temperature °C	Reference
evt	100	CAACACTGGATGATCTCAG	56	[15]
		CCCCCTCAACTGCTAATA
ial	320	CTGGATGGTATGGTGAGG	60	[18]
		GGAGGCCAACAATTATTTCC
ipaBCD	500	GCTATAGCAGTGACATG	59	[15]
		ACGAGTTCGAAGCACTC
ipaH	933	CTCGGCACGTTTTAATAGTCTGG	59	[19]
		GTGGAGAGCTGAAGTTTCTCTGC
set1A	309	TCACGCTACCATCAAAGA	57	[18]
		TATCCCCCTTTGGTGGTA
set1B	147	GTGAACCTGCTGCCGATATC	57	[18]
		ATTAGTGGATAAAAATGACG
sen/ospD3	799	ATGTGCCTGCTATTATTTAT	52	[18]
		CATAATAATAAGCGGTCAGC
virF	618	TCAGGCAATGAAACTTTGAC	60	[19]
		TGGGCTTGATATTCCGATAAGTC
uidA	1487	ATGCCAGTCCAGCGTTTTTGC	54	[20]
		AAAGTGTGGGTCAATAATCAGGAAGTG
invE	766	CGATAGATGGCGAGAAATTATATCCCG	56	[20]
		CGATCAAGAATCCCTAACAGAAGAATCAC