HPLC-DAD Analysis, Antileishmanial, Antiproliferative, and Antibacterial Activities of Lacistema pubescens: An Amazonian Medicinal Plant

Table 1

Antileishmanial and antiproliferative activity of the methanolic extract and fractions of L. pubescens.

Samples	IC50 values (µg/mL)^b
	Antileishmanial activity (promastigote forms)^a				Antiproliferative activity
	La	Lb	Lm	Lc	HL60	Jurkat
Methanolic extract	3.9; (±0.7)	45.6; (±2.1)	>100	>100	47.2; (±13.08)	>100
Hexane	3.5; (±0.9)	17.0; (±0.07)	4.2; (±0.8)	>100	39.7; (±1.37)	>100
Ethyl acetate	>100	>100	>100	>100	>100	>100
Hydromethanol	>100	>100	>100	>100	>100	>100
Amphotericin B^c	0.4; (±0.05)	0.30; (±0.09)	0.3; (±0.09)	1.9; (±0.25)	—	—
Etoposide^d	—^e	—	—	—	0.02; (±0.01)	3.0; (±2.13)

Organism key: ^aLa: Leishmania amazonensis; Lb: Leishmania braziliensis; Lm: Leishmania major; Lc: Leishmania chagasi.
values (concentrations inhibiting cell growth by 50%). Data are presented as median and 95% confidence interval (in parentheses).