EBV, HCMV, HHV6, and HHV7 Screening in Bone Marrow Samples from Children with Acute Lymphoblastic Leukemia
Figure 1
The PCRs limit of detection. Serial dilutions of DNA from control cell line and plasmid were PCR amplified in order to know the lower limit of detection of our screening test. For EBV (a), the limit of detection is expressed in number of cellular and viral genomes (from Raji cell line). For HCMV (b), HHV6 (c), and HHV7 (d) the limits of detection are expressed as plasmid copies. Lower limits of detection are indicated with a white arrowhead. White arrows point to the number of cells or plasmid copies that were used for viral detection in ALL samples. The sizes of PCR products are indicated. Black arrowheads point to residual primers.
