EBV, HCMV, HHV6, and HHV7 Screening in Bone Marrow Samples from Children with Acute Lymphoblastic Leukemia
Figure 2
EBV screening. Image of seven representative samples showing that all of them were EBV negative by first round PCR (a) and positivity was only detected by nested PCR in 10 (14%; showing three) samples (b). Raji and Ramos (Ram) cell lines were used as positive and negative controls, respectively. A reaction without DNA was routinely run (−). (c) Amplification of β -actin endogenous gene. The sizes of PCR products are indicated. The black arrowhead points to residual primers.