BioMed Research International / 2014 / Article / Tab 1 / Research Article
Detection of Carbapenemase-Producing Enterobacteriaceae in the Baltic Countries and St. Petersburg Area Table 1 (a) Primers for the detection of carbapenemase-encoding genes. (b) Primers and probes used for multiplex real-time PCR detection of classes 1, 2, and 3 integrons.
(a) Reaction number Primers Oligonucleotide sequence (5′-3′) Volume for reaction PCR IMP (forward) GAATAGRRTGGCTTAAYTCTC
2.5
L IMP (reverse) CCAAACYACTASGTTATC 2.5
L VIM (forward) GTTTGGTCGCATATCGCAAC 0.25
L VIM (reverse) AATGCGCAGCACCAGGATAG 0.25
L GIM (forward) TCAATTAGCTCTTGGGCTGAC 0.25
L GIM (reverse) CGGAACGACCATTTGAATGG 0.25
L PCR NDM (forward) CAATATTATGCACCCGGTCG 0.5
L NDM (reverse) ATCATGCTGGCCTTGGGGAA 0.5
L OXA 48 (forward) GCGTGGTTAAGGATGAACAC 0.5
L OXA 48 (reverse) CATCAAGTTCAACCCAACCG 0.5
L PCR KPC (forward) GGCAGTCGGAGACAAAACC 0.5
L KPC (reverse) CCCTCGAGCGCGAGTCTA 0.5
L
(b) Primers/probes Oligonucleotide sequence (5′-3′) Location intI 1-LC1GCCTTGATGTTACCCGAGAG intI 1intI 1-LC5GATCGGTCGAATGCGTGT intI 2-LC2TGCTTTTCCCACCCTTACC intI 2intI 2-LC3GACGGCTACCCTCTGTTATCTC intI 3-LC1GCCACCACTTGTTTGAGGA intI 3intI 3-LC2GGATGTCTGTGCCTGCTTG intI 1-probeATTCCTGGCCGTGGTTCTGGGTTTT intI 1intI 2-probeTGGATACTCGCAAACAAGTTATTTTTACGCTG intI 2intI 3-probeCGCCACTCATTCGCCACCCA intI 3
