Research Article

Origanum dictamnus Oil Vapour Suppresses the Development of Grey Mould in Eggplant Fruit In Vitro

Table 1

The experimental design of the volatiles impacts of dittany essential oil (DIT; 0–50–100–250 μL/L) on gray mould (Botrytis cinerea) development in vitro (PDA medium) or in vivo (eggplant fruit). In sustained vapour enrichment, fruits (or plates) were inoculated with B. cinerea and then exposed to vapours. A second sample of fruits (or plates) was preexposed to vapours, inoculated with fungi, and transferred/stored to “ambient air” (AA; control). Treatments were maintained throughout at 12°C and 95% RH.

ConditionsTreatmentSection

In vitro
Colony growth (SE)at 20°C for 2 h, at 12°C for 5 dInoc.-5 d in VOL Section 2.3
Colony growth,
transfer (SE)
at 20°C for 2 h, at 12°C for 5 d, at 12°C for 5 dInoc.-5 d in VOL-transfer in PDA-5 d in AASection 2.3
Colony growth (ME)at 20°C for 2 h, at 12°C for 5 d, at 12°C for 5 d5 d in VOL-inoc.-5 d in AASection 2.3
Spore production (SE)at 12°C for 14 dInoc.-14 d in VOLSection 2.6
Spore production (ME)at 12°C for 5 d, at 12°C for 14 d5 d in VOL-inoc.-14 d in AA Section 2.6
Spore germination (SE)at 12°C for 14 d, at 12°C for 24 hInoc.-14 d in VOL-Inoc. in PDA-24 h in AA Section 2.7
Spore germination (ME)at 12°C for 5 d, at 12°C for 14 d, at 12°C for 24 h5 d in VOL-inoc.-14 d in AA-Inoc in PDA-24 h in AASection 2.7
In vivo
Lesion growth (SE)at 20°C for 2 h, at 12°C for 14 dInoc.-7 d in VOLSection 2.4(a)
Lesion growth (ME)at 20°C for 2 h, at 12°C for 5 d, at 12°C for 7 d5 d in VOL-inoc.-7 d in AASection 2.4(b)
Spore production (SE)at 12°C for 14 dInoc.-14 d in VOLSection 2.5
Spore production (ME)at 12°C for 5 d, at 12°C for 14 d5 d in VOL-inoc.-14 d in AA Section 2.5
Spore germination (SE)at 12°C for 14 d, at 12°C for 24 h Inoc.-14 d in VOL-Inoc. in PDA-24 h in AA Section 2.6
Spore germination (ME)at 12°C for 5 d, at 12°C for 14 d, at 12°C for 24 h5 d in VOL-inoc.-14 d in AA-Inoc in PDA-24 h in AASection 2.6

VOL: volatiles; SE: sustained effect; ME: memory effect; PDA: potato dextrose agar; Inoc.: inoculation; AA: ambient air.
Letters a, b under the “section” heading correspond with sections in the text.