Review Article

An Historical Perspective on How Advances in Microscopic Imaging Contributed to Understanding the Leishmania Spp. and Trypanosoma cruzi Host-Parasite Relationship

Figure 8

Field-emission scanning electron microscopy of mouse hearts infected with T. cruzi. Thick paraffin embedded sections of mouse hearts infected with Y strain metacyclic trypomastigotes were deparaffinized and processed for field emission scanning electron microscopy [1821]. Briefly, paraffin was removed by melting the sections block and then deparaffinized with xylol and ethanol. Next, heart muscle sections cut with a razor blade were dehydrated in an ethanol series, subjected to critical-point drying, and gold sputtered. (a) Amastigotes (red), scale bar, 4 μm; (b) trypomastigotes (blue), scale bar, 10 μm.
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