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BioMed Research International
Volume 2014, Article ID 571302, 10 pages
Clinical Study

Aberrant Frequency of IL-10-Producing B Cells and Its Association with Treg/Th17 in Adult Primary Immune Thrombocytopenia Patients

1Department of Haematology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China
2Department of Haematology, Jinshan Hospital, Fudan University, Shanghai 201508, China
3Department of Internal Medicine, John A. Burns School of Medicine, University of Hawaii, HI 96813, USA
4Department of Cardiothoracic Surgery, Tongji Hospital, Tongji University, Shanghai 200065, China
5Research Centre, Zhongshan Hospital, Fudan University, Shanghai 200032, China

Received 27 February 2014; Revised 20 May 2014; Accepted 3 June 2014; Published 26 June 2014

Academic Editor: Harry W. Schroeder

Copyright © 2014 Fanli Hua et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Regulatory B cells (Breg) are a distinct B cell subset with immunoregulatory properties. Pivotal to Breg function is interleukin-10. This study was to investigate the role of IL-10-producing B cell (B10) and its association with Treg and Th17 subsets in immune thrombocytopenia (ITP) patients. Methods. Peripheral blood mononuclear cells from ITP patients and controls were stimulated with PMA, ionomycin, and Brefeldin A. The frequencies of CD19+IL-10+ B cells, CD3+CD4+IL-17+ Th17 cells, and CD4+CD25hiFoxp3+ Treg cells were analyzed by flow cytometry. The mRNA expression of Foxp3 and RORγt was detected by real-time quantitative PCR. Results. The number of B10 cells was elevated in ITP patients. After first-line therapies, it remained at high level in patients who achieved complete or partial response but decreased in those who acquired no response. There was a positive correlation between B10 cells and Tregs in ITP both before and after therapies. The ratio of Treg/Th17 decreased in ITP, and it strongly correlated with B10 cells. Conclusions. The frequency of B10 cells is elevated in ITP and it correlates with both the Tregs counts and the Treg/Th17 ratio. B10 cells to regulate functional T cell subsets might be impaired in patients with ITP.