572183.fig.005a
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572183.fig.005b
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572183.fig.005c
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Figure 5: (a) Marking of vascular walls with immunohistochemistry for von Willebrand factor. Polyurethane-dressed wound on the left and human-amniotic-membrane-dressed wound on the right (300-fold magnification, scale bar: 50 μm) on the tenth postoperative day. The arrows point exemplarily to vessel walls that are seen as circular structures with a brownish color because of staining with primary and secondary antibodies. (b) Marking of proliferating cells by immunohistochemistry for Ki-67. Polyurethane-dressed wound on the left and human-amniotic-membrane-dressed wound on the right (300-fold magnification, scale bar: 50 μm) on the seventh postoperative day. The arrows point exemplarily to proliferating cells that have a brownish color because of the staining with primary and secondary antibodies. (c) Marking of a basement membrane by immunohistochemistry for laminin. Polyurethane-dressed wound on the left and human-amniotic-membrane-dressed wound on the right (300-fold magnification, scale bar: 50 μm) on the tenth postoperative day. Note the complete integrity of the basement membrane in the HAM example.