BioMed Research International / 2014 / Article / Tab 2

Research Article

C-Terminal Domain Swapping of SSB Changes the Size of the ssDNA Binding Site

Table 2

ssDNA binding properties of KpSSB, StSSB, PaSSB, KpSSBnStSSBc, and KpSSBnPaSSBc as analyzed by EMSA.

ProteinDNA[Protein]50 (nM)Complex number

KpSSBdT20ND0
dT25200 ± 201
dT35220 ± 301
dT45100 ± 101
dT50110 ± 201
dT55100 ± 202
dT60100 ± 102

StSSBdT15650 ± 1201
dT20450 ± 801
dT30420 ± 601
dT40420 ± 801
dT45440 ± 602
dT50440 ± 502

PaSSBdT20ND0
dT251700 ± 2501
dT35950 ± 1801
dT45780 ± 1601
dT55820 ± 901
dT60810 ± 1102
dT65550 ± 702

KpSSBnStSSBcdT15260 ± 601
dT20110 ± 201
dT40120 ± 201
dT45160 ± 202

KpSSBnPaSSBcdT20ND0
dT25390 ± 601
dT40220 ± 301
dT55230 ± 301
dT60230 ± 302

[Protein]50 was calculated from the titration curves of EMSA by determining the concentration of the protein (μM) needed to achieve the midpoint value for input ssDNA binding. For some oligonucleotides, input ssDNA binding was the sum of the intensities from the two separate ssDNA-protein complexes. Errors are standard deviations determined by three independent titration experiments.