Cellular Levels of Oxidative Stress Affect the Response of Cervical Cancer Cells to Chemotherapeutic Agents

Figure 4

Pretreatment of CaSki and SiHa cells with NAC decreases oxidative stress and protects them from death induced by DOX (a) and cisplatin (b), while pretreatment with BSO increases ROS (c) and sensitizes cells to cell death induced by DOX (d) and cisplatin (e). (a and b) SiHa and CaSki cells (1 × 10⁴ cells per well) were seeded into 96-well plate and then pretreated with the indicated concentrations of NAC for 4 h followed by treatment with DOX for 48 h (a) or cisplatin for 48 h (b). The viability of cells untreated with drugs in the presence or absence of NAC cells was set at 100%. (c) 10⁵ CaSki or SiHa cells were treated with 10 or 20 μM BSO for 24 h and ROS measurements were performed as described in Figure 3(a). (d and e) Cells (1 × 10⁴ cells per well) were seeded onto a 96-well plate and then pretreated with indicated concentrations of BSO for 24 h followed by treatment with DOX (d) or cisplatin (e) for 48 h. Viability was assessed by crystal violet staining. The viability of cells untreated with drugs in the presence or absence of BSO was set at 100%.

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