Cellular Levels of Oxidative Stress Affect the Response of Cervical Cancer Cells to Chemotherapeutic Agents

Figure 6

Treatment of CaSki and SiHa cells with DOX increases the level of ROS detected by DHE (a), while treatment with cisplatin increases the level of ROS detected by DCFDA (b). 10⁵ CaSki or SiHa cells were treated with 0.5 or 1 μM DOX for 2 h (a) or with 60 or 100 μM cisplatin for 4 h (b). DCFDA or DHE was added to the media to a final concentration of 10 μM and cells were incubated in the dark at 37°C for 30 minutes. Cells were then washed, resuspended in 1x PBS, and analysed using flow cytometry. A total of 10 000 events were measured per sample. DCFDA was detected in the FL-1 channel, while DHE was detected in the FL-2 channel. Bar graphs show triplicate measurements of the mean fluorescence intensity of DCFDA or DHE in SiHa and CaSki cells, expressed as 100% of the value observed in untreated cells.

(a)

(b)