The Molecular Mechanisms of Tanshinone IIA on the Apoptosis and Arrest of Human Esophageal Carcinoma Cells
Figure 2
TanIIA inhibits the growth of esophageal cancer cells in a time- and concentration-dependent manner. (a) EC-1 cell; (c) ECa-109 cell. Cells were seeded onto 96-well plate at 5 × 103 cells/well and were treated with increasing concentrations of 0.3, 0.6, 1.3, 2.5, and 5.0 μg/mL TanIIA for 24, 48 h, and 72 h, respectively. Cell viability was determined by the CCK-8 assay. Results are performed by independent experiments in triplicate. (b) EC-1 cell; (d) ECa-109 cell. Cells were seeded onto 6-well plate at 3000/well and were treated with concentrations of 0.6 and 1.3 μg/mL TanIIA for 48 h and maintained for 5 days, respectively. Colony formations were fixed by methanol and acetic acid (3 : 1) and stained with Giemsa. Data represent means ± SD. * versus control.
