Figure 5: Agmatine inhibits Zymosan-induced NF-κB activation and DNA binding activity in lung. 6 h after ZYM injection with or without agmatine treatments, mice were exsanguinated and their lungs were removed. (a) Western blot was performed to detect p-IκB-α and IκB-α in cytoplasm and NF-κB p65 in nucleus. Expressions of β-actin and LaminB1 were shown as loading controls. (b) DNA binding activity of NF-κB p65 was examined by a TransAM p65 transcription factor ELISA kit. Each bar represents the mean ± SD of 8 mice. compared with NS group. # compared with Zymosan group.