Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom
Figure 3
Enzymatic characterization of BpirSP-39. (a) Fibrinogenolytic activity demonstrating the degradation of the α and β chains of bovine fibrinogen. (b) Fibrinolytic activity. The data are expressed in millimeters. (c) Gelatinase activity stained with Coomassie R-250. (d) Amidolytic activity on chromogenic substrate BApNA. The crude venom of B. pirajai was used as positive control and the dilution buffer of the sample as negative control. (*) Values significantly different from the negative control and (#) values significantly different from positive control .
