Effect of Hypoxia on the Calcium and Magnesium Content, Lipid Peroxidation Level, and Ca²⁺-ATPase Activity of Syncytiotrophoblast Plasma Membranes from Placental Explants

Table 3

Lipid peroxidation (as TBARS), PMCA, and and content of syncytiotrophoblast plasma membranes from placental explants cultured under either normoxia or hypoxia conditions.

Measured parameters	BM		MVM
	Normoxia	Hypoxia	Normoxia	Hypoxia
TBARS; nmol malondialdehyde/mg prot	0.72 ± 0.04; (4)	1.42 ± 0.09; (4)**	0.63 ± 0.03; (4)	1.48 ± 0.18; (4)**
PMCA activity; nmol Pi/mg prot·min	51.0 ± 6.9; (5)	13.7 ± 1.9; (5)**	93 ± 5.6; (5)	34 ± 2.9; (5)**
Membrane Ca^2+ content; µmol/g prot	56 ± 3; (5)	82 ± 4; (6)**	66 ± 5; (6)	101 ± 8; (6)*
Membrane Mg^2+ content; µmol/g prot	54 ± 4; (6)	51 ± 2; (6)	57 ± 3; (6)	58 ± 4; (6)

Preincubations were carried out for 18 h at 37°C. Values are expressed as the means ± S.E. for the indicated between parentheses.
versus normoxia.
versus normoxia.