Molecular Analysis of Ciprofloxacin Resistance Mechanisms in Malaysian ESBL-Producing Klebsiella pneumoniae Isolates and Development of Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations
Figure 2
Agarose gel electrophoresis image of PCR products generated from duplex MAMA-PCR assays (a) gyrA83 + parC80 and (b) gyrA87 + parC84. Identification of each target was based on the expected product size. Lanes 1-2 represent PCR products generated in the presence of the wild-type alleles. Lanes 3–6 are examples of products generated in case of mutations in one gene or in both target genes. M: DNA molecular size marker (100 bp DNA Ladder, Solis BioDyne, Estonia).
