Rational Design of a Triple Reporter Gene for Multimodality Molecular Imaging
Figure 2
The firefly luciferase and tTKSR39 activity exhibited by H1299 cells transiently transfected with different gene constructs. The H1299 cells were cotransfected with CMV-rl and fl-harboring gene constructs. The tTK activity normalized with CMV-EGFP was expressed as cell to medium accumulation/g protein (a). Values for FLUC activity was normalized with RLUC activity and expressed as relative light units (RLU)/g protein/RLUC activity (b). All the experiments () were done in triplicate. *.
