BioMed Research International / 2014 / Article / Tab 1 / Research Article
A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157:H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR Table 1 Primers and probes used in qPCR assays.
Target gene Sequence (5′-3′) Fragment size Reference hlyA Forward primer CATGGCACCACCAGCATCT 64 bp Reverse primer ATCCGCGTGTTTCTTTTCGA [24 ] Probe FAM-CGCCTGCAAGTCCTAAGACGCCA-TAMRA rfbE Forward primer TTTCACACTTATTGGATGGTCTCAA 88 bp Reverse primer CGATGAGTTTATCTGCAAGGTGAT [26 ] Probe VIC-AGGACCGCAGAGGAAAGAGAGGAATTAAGG-TAMRA fliC H7Forward primer CCACGACAGGTCTTTATGATCTGA 96 bp Reverse primer CAACTGTGACTTTATCGCCATTCC [26 ] Probe FAM-CCGAAAATACCTTGTTAACTACCGATGCTGC-BHQ
