Research Article

Phenotypic Characterization of Mycoplasma synoviae Induced Changes in the Metabolic and Sensitivity Profile of In Vitro Infected Chicken Chondrocytes

Figure 2

An example of the compared carbon source metabolic activities of CCH and CCH exposed to M. synoviae WVU 1853 membrane fraction (MSm) in PMM 1 containing different carbon and nitrogen sources. Kinetic data were collected using the Biolog Omnilog instrument and software. The curves show the time course (horizontal axis) of the amount of purple colour formed from tetrazolium dye reduction (vertical axis) in each of the 96 wells. All respiratory signals were previously corrected to the respiration of CCH in well A1, containing no supplements. Data from CCH are shown in red, from CCH+MSm in green, and yellow is the overlapping of the two kinetic curves. Wells where differences between respiratory signals reached a cutoff value (500) are encircled white and are of two types: (i) C sources more rapidly metabolized by CCH compared to CCH+MSm are shown in red (A12, b-gentibiose); (ii) C sources more rapidly metabolized by CCH+MSm compared CCH are shown in green (C5, D-mannose). Wells that are not encircled represent compounds where metabolism was not detected or are metabolized equally both in CCH and CCH+MSm and are shown in yellow (C4, mannan; C10, sucrose).
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